xbai agei restriction sites (New England Biolabs)
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xbai agei restriction sites
Xbai Agei Restriction Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6773 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/restriction+sites+agei/XbaI/pm41002377-55-6-9
Average 99 stars, based on 6773 article reviews
Xbai Agei Restriction Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6773 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/restriction+sites+agei/XbaI/pm41002377-55-6-9
Average 99 stars, based on 6773 article reviews
xbai agei restriction sites - by Bioz Stars,
2026-09
99/100 stars
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Plasmid Preparation:Article Title: Plastidial engineering with coupled farnesyl diphosphate pool reconstitution and enhancement for sesquiterpene biosynthesis in tomato fruit. Article Snippet: Sesquiterpenes represent a large class of terpene compounds found in plants with broad applications such as pharmaceuticals and biofuels.. The plastidial MEP pathway in ripening tomato fruit is naturally optimized to provide the 5-carbon isoprene building blocks of all terpenes for production of the tetraterpene pigment lycopene and other carotenoids, making it an excellent plant system to be engineered for production of high-value terpenoids.. We reconstituted and enhanced the pool of sesquiterpene precursor farnesyl diphosphate (FPP) in plastids of tomato fruit by overexpressing the fusion gene DXS-FPPS encoding a fusion protein of 1-deoxy-D-xylulose 5-phosphate synthase (DXS) linked with farnesyl diphosphate synthase (originally called farnesyl pyrophosphate synthase, and abbreviated as FPPS) under the control of fruit-ripening specific polygalacturonase (PG) promoter concomitant with substantial reduction in lycopene content and large production of FPP-derived squalene. Article Title: Molecular basis for the increased membrane fusion activity of the Ebola virus glycoprotein A82V variant from the 2013-2016 epidemic: insights from simulations and experiments Article Snippet: For GPΔmuc expression on pseudovirus, the plasmid pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain (Addgene plasmid # 86021) was first modified by site-directed mutagenesis (GeneScript USA Inc., Piscataway, NJ) to change the C-terminal amber stop codon to an ochre stop codon, to prevent readthrough and incorporation of trans-Cyclooct-2-en-L-Lysine axial isomer (TCO*A) at this site during the production of pseudovirus for smFRET imaging. .. This plasmid was further modified to introduce amber stop codons at specific sites for fluorophore attachment, namely N550TAG (asparagine to amber stop codon at GPΔmuc amino acid 550) followed by K617TAG (lysine to amber stop codon at GPΔmuc amino acid 617) by overlap PCR using PfuUltra II Fusion HS DNA Polymerase (Agilent Technologies Inc., SantaClara, CA), the following primers, and the Article Title: Engineering of tomato type VI glandular trichomes for trans-chrysanthemic acid biosynthesis, the acid moiety of natural pyrethrin insecticides. Article Snippet: Glandular trichomes, known as metabolic cell factories, have been proposed as highly suitable for metabolically engineering the production of plant high-value specialized metabolites.. Natural pyrethrins, found only in Dalmatian pyrethrum (Tanacetum cinerariifolium), are insecticides with low mammalian toxicity and short environmental persistence.. Type I pyrethrins are esters of the monoterpenoid trans-chrysanthemic acid with one of the three rethrolone-type alcohols. Cloning:Article Title: Plastidial engineering with coupled farnesyl diphosphate pool reconstitution and enhancement for sesquiterpene biosynthesis in tomato fruit. Article Snippet: Sesquiterpenes represent a large class of terpene compounds found in plants with broad applications such as pharmaceuticals and biofuels.. The plastidial MEP pathway in ripening tomato fruit is naturally optimized to provide the 5-carbon isoprene building blocks of all terpenes for production of the tetraterpene pigment lycopene and other carotenoids, making it an excellent plant system to be engineered for production of high-value terpenoids.. We reconstituted and enhanced the pool of sesquiterpene precursor farnesyl diphosphate (FPP) in plastids of tomato fruit by overexpressing the fusion gene DXS-FPPS encoding a fusion protein of 1-deoxy-D-xylulose 5-phosphate synthase (DXS) linked with farnesyl diphosphate synthase (originally called farnesyl pyrophosphate synthase, and abbreviated as FPPS) under the control of fruit-ripening specific polygalacturonase (PG) promoter concomitant with substantial reduction in lycopene content and large production of FPP-derived squalene. Article Title: Engineering of tomato type VI glandular trichomes for trans-chrysanthemic acid biosynthesis, the acid moiety of natural pyrethrin insecticides. Article Snippet: Glandular trichomes, known as metabolic cell factories, have been proposed as highly suitable for metabolically engineering the production of plant high-value specialized metabolites.. Natural pyrethrins, found only in Dalmatian pyrethrum (Tanacetum cinerariifolium), are insecticides with low mammalian toxicity and short environmental persistence.. Type I pyrethrins are esters of the monoterpenoid trans-chrysanthemic acid with one of the three rethrolone-type alcohols. Modification:Article Title: Molecular basis for the increased membrane fusion activity of the Ebola virus glycoprotein A82V variant from the 2013-2016 epidemic: insights from simulations and experiments Article Snippet: For GPΔmuc expression on pseudovirus, the plasmid pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain (Addgene plasmid # 86021) was first modified by site-directed mutagenesis (GeneScript USA Inc., Piscataway, NJ) to change the C-terminal amber stop codon to an ochre stop codon, to prevent readthrough and incorporation of trans-Cyclooct-2-en-L-Lysine axial isomer (TCO*A) at this site during the production of pseudovirus for smFRET imaging. .. This plasmid was further modified to introduce amber stop codons at specific sites for fluorophore attachment, namely N550TAG (asparagine to amber stop codon at GPΔmuc amino acid 550) followed by K617TAG (lysine to amber stop codon at GPΔmuc amino acid 617) by overlap PCR using PfuUltra II Fusion HS DNA Polymerase (Agilent Technologies Inc., SantaClara, CA), the following primers, and the Introduce:Article Title: Molecular basis for the increased membrane fusion activity of the Ebola virus glycoprotein A82V variant from the 2013-2016 epidemic: insights from simulations and experiments Article Snippet: For GPΔmuc expression on pseudovirus, the plasmid pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain (Addgene plasmid # 86021) was first modified by site-directed mutagenesis (GeneScript USA Inc., Piscataway, NJ) to change the C-terminal amber stop codon to an ochre stop codon, to prevent readthrough and incorporation of trans-Cyclooct-2-en-L-Lysine axial isomer (TCO*A) at this site during the production of pseudovirus for smFRET imaging. .. This plasmid was further modified to introduce amber stop codons at specific sites for fluorophore attachment, namely N550TAG (asparagine to amber stop codon at GPΔmuc amino acid 550) followed by K617TAG (lysine to amber stop codon at GPΔmuc amino acid 617) by overlap PCR using PfuUltra II Fusion HS DNA Polymerase (Agilent Technologies Inc., SantaClara, CA), the following primers, and the Polymerase Chain Reaction:Article Title: Molecular basis for the increased membrane fusion activity of the Ebola virus glycoprotein A82V variant from the 2013-2016 epidemic: insights from simulations and experiments Article Snippet: For GPΔmuc expression on pseudovirus, the plasmid pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain (Addgene plasmid # 86021) was first modified by site-directed mutagenesis (GeneScript USA Inc., Piscataway, NJ) to change the C-terminal amber stop codon to an ochre stop codon, to prevent readthrough and incorporation of trans-Cyclooct-2-en-L-Lysine axial isomer (TCO*A) at this site during the production of pseudovirus for smFRET imaging. .. This plasmid was further modified to introduce amber stop codons at specific sites for fluorophore attachment, namely N550TAG (asparagine to amber stop codon at GPΔmuc amino acid 550) followed by K617TAG (lysine to amber stop codon at GPΔmuc amino acid 617) by overlap PCR using PfuUltra II Fusion HS DNA Polymerase (Agilent Technologies Inc., SantaClara, CA), the following primers, and the Mutagenesis:Article Title: Molecular basis for the increased membrane fusion activity of the Ebola virus glycoprotein A82V variant from the 2013-2016 epidemic: insights from simulations and experiments Article Snippet: For GPΔmuc expression on pseudovirus, the plasmid pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain (Addgene plasmid # 86021) was first modified by site-directed mutagenesis (GeneScript USA Inc., Piscataway, NJ) to change the C-terminal amber stop codon to an ochre stop codon, to prevent readthrough and incorporation of trans-Cyclooct-2-en-L-Lysine axial isomer (TCO*A) at this site during the production of pseudovirus for smFRET imaging. .. This plasmid was further modified to introduce amber stop codons at specific sites for fluorophore attachment, namely N550TAG (asparagine to amber stop codon at GPΔmuc amino acid 550) followed by K617TAG (lysine to amber stop codon at GPΔmuc amino acid 617) by overlap PCR using PfuUltra II Fusion HS DNA Polymerase (Agilent Technologies Inc., SantaClara, CA), the following primers, and the Expressing:Article Title: Molecular basis for the increased membrane fusion activity of the Ebola virus glycoprotein A82V variant from the 2013-2016 epidemic: insights from simulations and experiments Article Snippet: For GPΔmuc expression on pseudovirus, the plasmid pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain (Addgene plasmid # 86021) was first modified by site-directed mutagenesis (GeneScript USA Inc., Piscataway, NJ) to change the C-terminal amber stop codon to an ochre stop codon, to prevent readthrough and incorporation of trans-Cyclooct-2-en-L-Lysine axial isomer (TCO*A) at this site during the production of pseudovirus for smFRET imaging. .. This plasmid was further modified to introduce amber stop codons at specific sites for fluorophore attachment, namely N550TAG (asparagine to amber stop codon at GPΔmuc amino acid 550) followed by K617TAG (lysine to amber stop codon at GPΔmuc amino acid 617) by overlap PCR using PfuUltra II Fusion HS DNA Polymerase (Agilent Technologies Inc., SantaClara, CA), the following primers, and the Article Title: Engineering of tomato type VI glandular trichomes for trans-chrysanthemic acid biosynthesis, the acid moiety of natural pyrethrin insecticides. Article Snippet: Glandular trichomes, known as metabolic cell factories, have been proposed as highly suitable for metabolically engineering the production of plant high-value specialized metabolites.. Natural pyrethrins, found only in Dalmatian pyrethrum (Tanacetum cinerariifolium), are insecticides with low mammalian toxicity and short environmental persistence.. Type I pyrethrins are esters of the monoterpenoid trans-chrysanthemic acid with one of the three rethrolone-type alcohols. Sequencing:Article Title: Molecular basis for the increased membrane fusion activity of the Ebola virus glycoprotein A82V variant from the 2013-2016 epidemic: insights from simulations and experiments Article Snippet: For GPΔmuc expression on pseudovirus, the plasmid pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain (Addgene plasmid # 86021) was first modified by site-directed mutagenesis (GeneScript USA Inc., Piscataway, NJ) to change the C-terminal amber stop codon to an ochre stop codon, to prevent readthrough and incorporation of trans-Cyclooct-2-en-L-Lysine axial isomer (TCO*A) at this site during the production of pseudovirus for smFRET imaging. .. This plasmid was further modified to introduce amber stop codons at specific sites for fluorophore attachment, namely N550TAG (asparagine to amber stop codon at GPΔmuc amino acid 550) followed by K617TAG (lysine to amber stop codon at GPΔmuc amino acid 617) by overlap PCR using PfuUltra II Fusion HS DNA Polymerase (Agilent Technologies Inc., SantaClara, CA), the following primers, and the Synthesized:Article Title: Molecular basis for the increased membrane fusion activity of the Ebola virus glycoprotein A82V variant from the 2013-2016 epidemic: insights from simulations and experiments Article Snippet: For GPΔmuc expression on pseudovirus, the plasmid pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain (Addgene plasmid # 86021) was first modified by site-directed mutagenesis (GeneScript USA Inc., Piscataway, NJ) to change the C-terminal amber stop codon to an ochre stop codon, to prevent readthrough and incorporation of trans-Cyclooct-2-en-L-Lysine axial isomer (TCO*A) at this site during the production of pseudovirus for smFRET imaging. .. This plasmid was further modified to introduce amber stop codons at specific sites for fluorophore attachment, namely N550TAG (asparagine to amber stop codon at GPΔmuc amino acid 550) followed by K617TAG (lysine to amber stop codon at GPΔmuc amino acid 617) by overlap PCR using PfuUltra II Fusion HS DNA Polymerase (Agilent Technologies Inc., SantaClara, CA), the following primers, and the Clone Assay:Article Title: Molecular basis for the increased membrane fusion activity of the Ebola virus glycoprotein A82V variant from the 2013-2016 epidemic: insights from simulations and experiments Article Snippet: For GPΔmuc expression on pseudovirus, the plasmid pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain (Addgene plasmid # 86021) was first modified by site-directed mutagenesis (GeneScript USA Inc., Piscataway, NJ) to change the C-terminal amber stop codon to an ochre stop codon, to prevent readthrough and incorporation of trans-Cyclooct-2-en-L-Lysine axial isomer (TCO*A) at this site during the production of pseudovirus for smFRET imaging. .. This plasmid was further modified to introduce amber stop codons at specific sites for fluorophore attachment, namely N550TAG (asparagine to amber stop codon at GPΔmuc amino acid 550) followed by K617TAG (lysine to amber stop codon at GPΔmuc amino acid 617) by overlap PCR using PfuUltra II Fusion HS DNA Polymerase (Agilent Technologies Inc., SantaClara, CA), the following primers, and the Variant Assay:Article Title: Molecular basis for the increased membrane fusion activity of the Ebola virus glycoprotein A82V variant from the 2013-2016 epidemic: insights from simulations and experiments Article Snippet: For GPΔmuc expression on pseudovirus, the plasmid pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain (Addgene plasmid # 86021) was first modified by site-directed mutagenesis (GeneScript USA Inc., Piscataway, NJ) to change the C-terminal amber stop codon to an ochre stop codon, to prevent readthrough and incorporation of trans-Cyclooct-2-en-L-Lysine axial isomer (TCO*A) at this site during the production of pseudovirus for smFRET imaging. .. This plasmid was further modified to introduce amber stop codons at specific sites for fluorophore attachment, namely N550TAG (asparagine to amber stop codon at GPΔmuc amino acid 550) followed by K617TAG (lysine to amber stop codon at GPΔmuc amino acid 617) by overlap PCR using PfuUltra II Fusion HS DNA Polymerase (Agilent Technologies Inc., SantaClara, CA), the following primers, and the Construct:Article Title: Engineering of tomato type VI glandular trichomes for trans-chrysanthemic acid biosynthesis, the acid moiety of natural pyrethrin insecticides. Article Snippet: Glandular trichomes, known as metabolic cell factories, have been proposed as highly suitable for metabolically engineering the production of plant high-value specialized metabolites.. Natural pyrethrins, found only in Dalmatian pyrethrum (Tanacetum cinerariifolium), are insecticides with low mammalian toxicity and short environmental persistence.. Type I pyrethrins are esters of the monoterpenoid trans-chrysanthemic acid with one of the three rethrolone-type alcohols. |